Moroccan Endemic Artemisia herba-alba Essential Oil: GC-MS Analysis and Antibacterial and Antifungal Investigation

In Morocco, the endemic Artemisia herba-alba is well known by its traditional uses and health benefits. The search for natural, safe, effective antibacterial antifungal agents from plants in high demand due to microbial fungal resistance conventional synthetic antibiotics drugs. this study, A. was collected region of Fez-Boulemane during periods March, June, September. Essential oils (EOs) were extracted aerial part plant hydrodistillation method. chemical constituents determined using GC-MS as analytical tools. antimicrobial activities different tested macrodilution results showed difference yields between three EOs (0.49, 1.74, 1.30% (mL/100 g)), respectively, their corresponding compositions. main revealed are higher contents oxygenated monoterpenes (84.7, 84.4, 81%), such cis chrysanthenyl acetate (30, 26.7, 27.6%), ?-thujone (23.2, 12.9, 15.4%), camphor (9.76, 14.3, 15.8%), chrysanthenone (2.4, 1, 14%), 1,8-cineole (1.5, 11.7, 11.8%), trans ?-dihydroterpineol (7.8, 7.2, 6.9%), ?-thujone (4.8, 3, 5.4%), sesquiterpenic davanone (3.9, 1.5, 1.4%), respectively. biological activities’ significant effects against four bacteria (E. coli, B. subtilis, S. aureus, M. luteus), with MIC values ranging 0.1 0.03% (v/v), interesting on both wood rot fungi examined (G. trabeum, P. placenta, C. puteana, versicolor) molds microorganisms (A. niger, digitatum, expansum), 0.2 (v/v) 0.4 June September EO samples more potent than those March. Our research findings quantitative variability compositions, which could be phenological stages, climatic conditions growth, harvesting periods. antimicrobial/antifungal provided might correlated contribution synergism effect all monoterpenes. These support possible application natural safe agents, an alternative drugs, enabling prevention treatment certain pathogenic infections food health, preservation alteration fungi.